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Improved definition of the mouse transcriptome via targeted RNA sequencing

Abstract:

Targeted RNA sequencing (CaptureSeq) uses oligonucleotide probes to capture RNAs for sequencing, providing enriched read coverage, accurate measurement of gene expression, and quantitative expression data. We applied CaptureSeq to refine transcript annotations in the current murine GRCm38 assembly. More than 23,000 regions corresponding to putative or annotated long noncoding RNAs (lncRNAs) and 154,281 known splicing junction sites were selected for targeted sequencing across five mouse tissu...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1101/gr.199760.115

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Institution:
University of Oxford
Department:
Oxford, MSD, Physiology Anatomy and Genetics
Role:
Author
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Publisher:
Cold Spring Harbor Laboratory Press Publisher's website
Journal:
Genome research Journal website
Volume:
26
Issue:
5
Pages:
705-716
Publication date:
2016-05-05
Acceptance date:
2016-02-23
DOI:
EISSN:
1549-5469
ISSN:
1088-9051
URN:
uuid:25f0d057-b677-4c55-8943-c392e2353084
Source identifiers:
623617
Local pid:
pubs:623617
Paper number:
5
Language:
English
Keywords:

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