A comparison of exogenous promoter activity at the ROSA26 locus using a (Phi)iC31 integrase mediated cassette exchange approach in mouse ES cells.
The activities of nine ubiquitous promoters (ROSA26, CAG, CMV, CMVd1, UbC, EF1(Alpha), PGK, chicken (Beta)-actin and MC1) have been quantified and compared in mouse embryonic stem cells. To avoid the high variation in transgene expression which results from uncontrolled copy number and chromosomal position effects when using random insertion based transgenic approaches, we have adopted a PhiC31 integrase mediated cassette exchange method for the efficient insertion of transgenes at single cop...Expand abstract
- Publication status:
- Peer review status:
- Peer reviewed
(Version of record, pdf, 378.3KB)
- Publisher copy:
- Local pid:
- Deposit date:
- Copyright holder:
- Chen et al
- Copyright date:
- Copyright 2011 Chen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- CC Attribution (CC BY)
If you are the owner of this record, you can report an update to it here: Report update to this record