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A comparison of exogenous promoter activity at the ROSA26 locus using a (Phi)iC31 integrase mediated cassette exchange approach in mouse ES cells.

Abstract:

The activities of nine ubiquitous promoters (ROSA26, CAG, CMV, CMVd1, UbC, EF1(Alpha), PGK, chicken (Beta)-actin and MC1) have been quantified and compared in mouse embryonic stem cells. To avoid the high variation in transgene expression which results from uncontrolled copy number and chromosomal position effects when using random insertion based transgenic approaches, we have adopted a PhiC31 integrase mediated cassette exchange method for the efficient insertion of transgenes at single cop...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1371/journal.pone.0023376

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
RDM Cardiovascular Medicine
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Human Genetics Wt Centre
Role:
Author
More from this funder
Funding agency for:
Bhattacharya, S
Grant:
083228
Wellcome Trust More from this funder
Publisher:
Public Library of Science Publisher's website
Journal:
PloS one Journal website
Volume:
6
Issue:
8
Article number:
e23376
Publication date:
2011-01-01
DOI:
EISSN:
1932-6203
ISSN:
1932-6203
Source identifiers:
172055

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