Journal article

A photo-CIDNP study of the active sites of Megasphaera elsdenii and Clostridium MP flavodoxins.

Abstract:: Megasphaera elsdenii and Clostridium MP flavodoxins have been investigated by photo-CIDNP techniques. Using time-resolved spectroscopy and external dyes carrying different charges it was possible to assign unambiguously the resonance lines in the NMR-spectra to tyrosine, tryptophan and methionine residues in the two proteins. The results show that Trp-91 in M.elsdenii and Trp-90 in Cl.MP flavodoxin are strongly immobilized and placed directly above the benzene subnucleus of the prosthetic group. The data further indicate that the active sites of the two flavodoxins are extremely similar.

Publication status:: Published

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APA Style

Moonen, C., Hore, P., Müller, F., Kaptein, R., & Mayhew, S. (1982). A photo-CIDNP study of the active sites of Megasphaera elsdenii and Clostridium MP flavodoxins. FEBS Letters, 149(1), 141–146.

MLA Style

Moonen, C., et al. “A Photo-CIDNP Study of the Active Sites of Megasphaera Elsdenii and Clostridium MP Flavodoxins.” FEBS Letters, vol. 149, no. 1, 1982, pp. 141–46.

Chicago Style

Moonen, C, P Hore, F Müller, R Kaptein, and S Mayhew. 1982. “A Photo-CIDNP Study of the Active Sites of Megasphaera Elsdenii and Clostridium MP Flavodoxins.” FEBS Letters 149 (1): 141–46.
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Publisher copy:: 10.1016/0014-5793(82)81090-9

Authors

+ Moonen, C More by this author

Role:: Author

+ Hore, P More by this author

Institution:: University of Oxford
Division:: MPLS
Department:: Chemistry
Sub department:: Physical & Theoretical Chem
Role:: Author

+ Müller, F More by this author

Role:: Author

+ Kaptein, R More by this author

Role:: Author

+ Mayhew, S More by this author

Role:: Author

Journal:: FEBS letters More from this journal
Volume:: 149
Issue:: 1
Pages:: 141-146
Publication date:: 1982-11-01
DOI:: 10.1016/0014-5793(82)81090-9
EISSN:: 1873-3468
ISSN:: 0014-5793

Language:: English
Keywords:: Binding Sites

Veillonellaceae

Light

Magnetic Resonance Spectroscopy

Flavoproteins

Flavodoxin

Species Specificity

Clostridium
Pubs id:: pubs:35568
UUID:: uuid:25676fd0-39de-435f-8104-b07b476ebbd6
Local pid:: pubs:35568
Source identifiers:: 35568
Deposit date:: 2012-12-19

Terms of use

Copyright date:: 1982

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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