Journal article
Envelope glycoprotein mobility on HIV-1 particles depends on the virus maturation state
- Abstract:
- Human Immunodeficiency Virus (HIV-1) assembles as immature particles which require the proteolytic cleavage of structural polyprotein Gag and the clustering of envelope glycoprotein Env for infectivity. The details of mechanisms underlying Env clustering remain unknown. Here we determine molecular dynamics of Env on the surface of individual HIV-1 particles using scanning Fluorescence Correlation Spectroscopy on a super-resolution STED microscope (sSTED-FCS). We find that Env undergoes a maturation-induced increase in mobility, highlighting diffusion as one cause for Env clustering. This mobility increase is dependent on Gag-interacting Env tail but not on changes in viral envelope lipid order. Diffusion of Env and other envelope incorporated proteins in mature HIV-1 is two orders of magnitude slower than in the plasma membrane, indicating that HIV-1 envelope is intrinsically a low mobility environment, mainly due to its high lipid order. Our results provide novel insights into dynamic properties of proteins on the surface of individual virus particles.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 1.8MB, Terms of use)
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- Publisher copy:
- 10.1038/s41467-017-00515-6
Authors
+ Engineering and Physical Sciences Research Counci
More from this funder
- Funding agency for:
- Waithe, D
- Grant:
- EP/L016052/1
+ John Fell Fund
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- Funding agency for:
- Chojnacki, J
- Eggeling, C
- Grant:
- 1905“Structure
- functionoftheperoxisomaltranslocon”
- 1905“Structure
- functionoftheperoxisomaltranslocon”
+ EPA Cephalosporin Fund
More from this funder
- Funding agency for:
- Chojnacki, J
- Eggeling, C
- Grant:
- functionoftheperoxisomaltranslocon”
- 1905“Structure
- functionoftheperoxisomaltranslocon”
- 1905“Structure
+ Deutsche Forschungsgemeinschaft
More from this funder
- Funding agency for:
- Chojnacki, J
- Eggeling, C
- Grant:
- 1905“Structure
- functionoftheperoxisomaltranslocon”
- functionoftheperoxisomaltranslocon”
- 1905“Structure
+ Wellcome Trust
More from this funder
- Funding agency for:
- Chojnacki, J
- Eggeling, C
- Grant:
- functionoftheperoxisomaltranslocon”
- 1905“Structure
- functionoftheperoxisomaltranslocon”
- 1905“Structure
- Publisher:
- Springer Nature
- Journal:
- Nature Communications More from this journal
- Volume:
- 8
- Article number:
- 545
- Publication date:
- 2017-09-15
- Acceptance date:
- 2017-06-30
- DOI:
- ISSN:
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2041-1723
- Keywords:
- Pubs id:
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pubs:710277
- UUID:
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uuid:24ebbfcc-d91d-4c0e-849a-592865487a3c
- Local pid:
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pubs:710277
- Source identifiers:
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710277
- Deposit date:
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2017-08-03
Terms of use
- Copyright holder:
- Chojnacki et al
- Copyright date:
- 2017
- Notes:
- This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
- Licence:
- CC Attribution (CC BY)
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