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Codon optimization can improve expression of human genes in Escherichia coli: A multi-gene study.

Abstract:

The efficiency of heterologous protein production in Escherichia coli (E. coli) can be diminished by biased codon usage. Approaches normally used to overcome this problem include targeted mutagenesis to remove rare codons or the addition of rare codon tRNAs in specific cell lines. Recently, improvements in technology have enabled cost-effective production of synthetic genes, making this a feasible alternative. To explore this option, the expression patterns in E. coli of 30 human short-chain ...

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Publication status:
Published

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Publisher copy:
10.1016/j.pep.2008.01.008

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Structural Genomics Consortium
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Organic Chemistry
Role:
Author
Journal:
Protein expression and purification More from this journal
Volume:
59
Issue:
1
Pages:
94-102
Publication date:
2008-05-01
DOI:
EISSN:
1096-0279
ISSN:
1046-5928
Language:
English
Keywords:
Pubs id:
pubs:34140
UUID:
uuid:245de3a5-6108-4c81-a3da-7de47e072d6e
Local pid:
pubs:34140
Source identifiers:
34140
Deposit date:
2012-12-19

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