Journal article
A mobile endocytic network connects clathrin-independent receptor endocytosis to recycling and promotes T cell activation
- Abstract:
- Endocytosis of surface receptors and their polarized recycling back to the plasma membrane are central to many cellular processes, such as cell migration, cytokinesis, basolateral polarity of epithelial cells and T cell activation. Little is known about the mechanisms that control the organization of recycling endosomes and how they connect to receptor endocytosis. Here, we follow the endocytic journey of the T cell receptor (TCR), from internalization at the plasma membrane to recycling back to the immunological synapse. We show that TCR triggering leads to its rapid uptake through a clathrin-independent pathway. Immediately after internalization, TCR is incorporated into a mobile and long-lived endocytic network demarked by the membrane-organizing proteins flotillins. Although flotillins are not required for TCR internalization, they are necessary for its recycling to the immunological synapse. We further show that flotillins are essential for T cell activation, supporting TCR nanoscale organization and signaling.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 11.2MB, Terms of use)
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- Publisher copy:
- 10.1038/s41467-018-04088-w
Authors
- Publisher:
- Nature Publishing Group
- Journal:
- Nature Communications More from this journal
- Volume:
- 9
- Article number:
- 1597
- Publication date:
- 2018-04-23
- Acceptance date:
- 2018-04-03
- DOI:
- ISSN:
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2041-1723
- Pmid:
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29686427
- Language:
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English
- Keywords:
- Pubs id:
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pubs:844918
- UUID:
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uuid:245361ad-27fe-4489-86a1-7a6b55e21d5a
- Local pid:
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pubs:844918
- Source identifiers:
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844918
- Deposit date:
-
2018-05-15
Terms of use
- Copyright holder:
- © Compeer, et al 2018
- Copyright date:
- 2018
- Notes:
- This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
- Licence:
- CC Attribution (CC BY)
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