Abolishment of morphology-based taxa and change to binomial species names: 2022 taxonomy update of the ICTV bacterial viruses subcommittee

Turner, D; Shkoporov, AN; Lood, C; Millard, AD; Dutilh, BE; Alfenas-Zerbini, P; van Zyl, LJ; Aziz, RK; Oksanen, HM; Poranen, MM; Kropinski, AM; Barylski, J; Brister, JR; Chanisvili, N; Edwards, RA; Enault, F; Gillis, A; Knezevic, P; Krupovic, M; Kurtböke, I; Kushkina, A; Lavigne, R; Lehman, S; Lobocka, M; Moraru, C

Journal article

Abolishment of morphology-based taxa and change to binomial species names: 2022 taxonomy update of the ICTV bacterial viruses subcommittee

Abstract:: Antibiotic resistance genes (ARGs) have been extensively observed in bacterial DNA, and more recently, in phage particles from various water sources and food items. The pivotal role played by ARG transmission in the proliferation of antibiotic resistance and emergence of new resistant strains calls for a thorough understanding of the underlying mechanisms. The aim of this study was to assess the suitability of the prototypical p-crAssphage, a proposed indicator of human fecal contamination, and the recently isolated crAssBcn phages, both belonging to the Crassvirales group, as potential indicators of ARGs. These crAss-like phages were evaluated alongside specific ARGs (blaTEM, blaCTX-M-1, blaCTX-M-9, blaVIM, blaOXA-48, qnrA, qnrS, tetW and sul1) within the total DNA and phage DNA fractions in water and food samples containing different levels of fecal pollution. In samples with high fecal load (>103 CFU/g or ml of E. coli or somatic coliphages), such as wastewater and sludge, positive correlations were found between both types of crAss-like phages and ARGs in both DNA fractions. The strongest correlation was observed between sul1 and crAssBcn phages (rho = 0.90) in sludge samples, followed by blaCTX-M-9 and p-crAssphage (rho = 0.86) in sewage samples, both in the phage DNA fraction. The use of crAssphage and crAssBcn as indicators of ARGs, considered to be emerging environmental contaminants of anthropogenic origin, is supported by their close association with the human gut. Monitoring ARGs can help to mitigate their dissemination and prevent the emergence of new resistant bacterial strains, thus safeguarding public health

Publication status:: Published

Peer review status:: Peer reviewed

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APA Style

Turner, D., Shkoporov, A. N., Lood, C., Millard, A. D., Dutilh, B. E., Alfenas-Zerbini, P., van Zyl, L. J., Aziz, R. K., Oksanen, H. M., Poranen, M. M., Kropinski, A. M., Barylski, J., Brister, J. R., Chanisvili, N., Edwards, R. A., Enault, F., Gillis, A., Knezevic, P., Krupovic, M., … Moraru, C. (2023). Abolishment of morphology-based taxa and change to binomial species names: 2022 taxonomy update of the ICTV bacterial viruses subcommittee. Archives of Virology, 168(2), 74–74.

MLA Style

Turner, D, et al. “Abolishment of Morphology-Based Taxa and Change to Binomial Species Names: 2022 Taxonomy Update of the ICTV Bacterial Viruses Subcommittee.” Archives of Virology, vol. 168, no. 2, 2023, pp. 74–74.

Chicago Style

Turner, D, AN Shkoporov, C Lood, et al. 2023. “Abolishment of Morphology-Based Taxa and Change to Binomial Species Names: 2022 Taxonomy Update of the ICTV Bacterial Viruses Subcommittee.” Archives of Virology 168 (2): 74–74.
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