Relative orientation between the beta-ionone ring and the polyene chain for the chromophore of rhodopsin in native membranes.

Journal article

Rotational resonance solid state nuclear magnetic resonance has been used to determine the relative orientation of the beta-ionone ring and the polyene chain of the chromophore 11-Z-retinylidene of rhodopsin in rod outer segment membranes from bovine retina. The bleached protein was regenerated with either 11-Z-[8,18-(13)C(2)]retinal or 11-Z-[8,16/17(13)C(2)]retinal, the latter having only one (13)C label at either of the chemically equivalent positions 16 and 17. Observation of (13)C selecti...

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Authors: Spooner, P; Sharples, J; Verhoeven, M; Lugtenburg, J; Glaubitz, C

• Publication status: Published
• Journal: Biochemistry
• Volume: 41
• Issue: 24
• Pages: 7549-7555
• Publication date: 2002-06-01
• DOI: 10.1021/bi020007o
• EISSN: 1520-4995
• ISSN: 0006-2960
• Language: English
• Keywords: Animals; Membrane Proteins; Retinoids; Nuclear Magnetic Resonance, Biomolecular; Terpenes; Norisoprenoids; Molecular Conformation; Rhodopsin; Carbon Isotopes; Cattle; Retinal Pigments; Polyenes; Rod Cell Outer Segment