Journal article
Evolution of a plant growth-regulatory protein interaction specificity
- Abstract:
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Specific protein–protein interactions (PPIs) enable biological regulation. However, the evolution of PPI specificity is little understood. Here we trace the evolution of the land-plant growth-regulatory DELLA–SLY1/GID2 PPI, revealing progressive increase in specificity of affinity of SLY1/GID2 for a particular DELLA form. While early-diverging SLY1s display relatively broad-range DELLA affinity, later-diverging SLY1s tend towards increasingly stringent affinity for a specific DELLA A’ form generated by the growth-promoting phytohormone gibberellin (GA). Our novel mutational strategy reveals amino acid substitutions contributing to the evolution of Arabidopsis thaliana SLY1 A’ specificity, also showing that routes permitting reversion to broader affinity became increasingly constrained over evolutionary time. We suggest that progressive affinity narrowing may be an important evolutionary driver of PPI specificity and that increase in SLY1/GID2-DELLA specificity enabled the enhanced flexibility of plant physiological environmental adaptation conferred by the GA-DELLA growth-regulatory mechanism.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Version of record, pdf, 15.0MB, Terms of use)
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- Publisher copy:
- 10.1038/s41477-023-01556-0
Authors
- Funder identifier:
- https://ror.org/01h0zpd94
- Grant:
- 32122065
- Funder identifier:
- https://ror.org/00cwqg982
- Grant:
- BB/S013741/1
- Publisher:
- Springer Nature
- Journal:
- Nature Plants More from this journal
- Volume:
- 9
- Issue:
- 12
- Pages:
- 2059-2070
- Publication date:
- 2023-10-30
- Acceptance date:
- 2023-09-27
- DOI:
- EISSN:
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2055-0278
- ISSN:
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2055-026X
- Pmid:
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37903985
- Language:
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English
- Keywords:
- Pubs id:
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1555950
- Local pid:
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pubs:1555950
- Deposit date:
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2025-02-14
- ARK identifier:
Terms of use
- Copyright holder:
- Ji et al.
- Copyright date:
- 2023
- Rights statement:
- Copyright © 2023, The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
- Licence:
- CC Attribution (CC BY)
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