The order and logic of CD4 versus CD8 lineage choice and differentiation in mouse thymus

Karimi, MM; Guo, Y; Cui, X; Pallikonda, HA; Horková, V; Wang, Y-F; Gil, SR; Rodriguez-Esteban, G; Robles-Rebollo, I; Bruno, L; Georgieva, R; Patel, B; Elliott, J; Dore, MH; Dauphars, D; Krangel, MS; Lenhard, B; Heyn, H; Fisher, AG; Štěpánek, O; Merkenschlager, M

Journal article

The order and logic of CD4 versus CD8 lineage choice and differentiation in mouse thymus

Abstract:: We aimed to investigate the effect of Commiphora gileadensis and Cynanchum radians extracts on T lymphocyte subsets. Fifty mice were divided into five groups: control group, C. gileadensis acetone-treated group, C. gileadensis sap-treated group, C. radians acetone-treated group, and C. radians methanolic-treated group. Blood was collected for flow cytometric analysis to measure various lymphocyte populations. A one-way ANOVA test was chosen to compare between-group differences. Data shows that there was a significant decrease in the number of total lymphocytes, CD3+, CD4+, and CD8+ cells in all mice treated with all extracts compared with controls. In addition, the CD4/CD8 ratio was significantly higher in mice treated with C. gileadensis acetone extract. Furthermore, there was an elevation of CD4+/CD25+ and CD8+/CD25+ cells in all groups treated with all extracts as compared with the control group. This study concluded that C. gileadensis and C. radians have immunomodulatory properties.Nuestro objetivo fue investigar el efecto de los extractos de Commiphora gileadensis y Cynanchum radians sobre los subconjuntos de linfocitos T. Cincuenta ratones se dividieron en cinco grupos: grupo control, grupo tratado con acetona de C. gileadensis, grupo tratado con savia de C. gileadensis, grupo tratado con acetona de C. radians y grupo tratado con metanol de C. radians. Se recolectó sangre para el análisis por citometría de flujo para medir diversas poblaciones de linfocitos. Se eligió una prueba ANOVA de un solo factor para comparar las diferencias entre grupos. Los datos muestran que hubo una disminución significativa en el número total de linfocitos, células CD3+, CD4+ y CD8+ en todos los ratones tratados con todos los extractos en comparación con los controles. Además, el ratio CD4/CD8 fue significativamente mayor en los ratones tratados con el extracto acetónico de C. gileadensis. Asimismo, hubo un aumento de las células CD4+/CD25+ y CD8+/CD25+ en todos los grupos tratados con todos los extractos en comparación con el grupo control. Este estudio concluyó que C. gileadensis y C. radians tienen propiedades inmunomoduladoras

Publication status:: Published

Peer review status:: Peer reviewed

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APA Style

Karimi, M. M., Guo, Y., Cui, X., Pallikonda, H. A., Horková, V., Wang, Y.-F., Gil, S. R., Rodriguez-Esteban, G., Robles-Rebollo, I., Bruno, L., Georgieva, R., Patel, B., Elliott, J., Dore, M. H., Dauphars, D., Krangel, M. S., Lenhard, B., Heyn, H., Fisher, A. G., … Merkenschlager, M. (2021). The order and logic of CD4 versus CD8 lineage choice and differentiation in mouse thymus. Nature Communications, 12(1), 99–99.

MLA Style

Karimi, MM, et al. “The Order and Logic of CD4 versus CD8 Lineage Choice and Differentiation in Mouse Thymus.” Nature Communications, vol. 12, no. 1, 2021, pp. 99–99.

Chicago Style

Karimi, MM, Y Guo, X Cui, et al. 2021. “The Order and Logic of CD4 versus CD8 Lineage Choice and Differentiation in Mouse Thymus.” Nature Communications 12 (1): 99–99.
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