Improved detection of methicillin-resistant Staphylococcus aureus using phenyl mannitol broth containing aztreonam and ceftizoxime.

Journal article

We tested a phenyl mannitol broth containing ceftizoxime and aztreonam (PHMB(+)) for detection of methicillin-resistant Staphylococcus aureus (MRSA) with reference MRSA strains and, subsequently, with clinical samples (n = 1,098). All reference MRSA strains induced color change in PHMB(+) after 24 to 72 h of incubation. In a clinical setting, 40 MRSA strains were detected with PHMB(+), compared with only 23 detected with a routine method. Thus, this selective broth significantly (P < 0.001) improved the rate of MRSA detection.

Authors: Wertheim, H; Verbrugh, H; van Pelt, C; de Man, P; van Belkum, A

• Publication status: Published
• Journal: Journal of clinical microbiology
• Volume: 39
• Issue: 7
• Pages: 2660-2662
• Publication date: 2001-07-01
• DOI: 10.1128/jcm.39.7.2660-2662.2001
• EISSN: 1098-660X
• ISSN: 0095-1137
• Language: English
• Keywords: Phenolsulfonphthalein; Aztreonam; Mannitol; Staphylococcus aureus; Ceftizoxime; Staphylococcal Infections; Methicillin Resistance; Monobactams; Cephalosporins; Humans; Bacteriological Techniques; Culture Media