Journal article

EXPRESSION IN ESCHERICHIA-COLI OF A CLAVAMINIC ACID SYNTHASE ISOZYME - A TRIFUNCTIONAL OXYGENASE INVOLVED IN CLAVULANIC ACID BIOSYNTHESIS

Abstract:: Purification and sequencing of clavaminic acid synthase (CAS) activities from S. clavuligerus SC2 provided evidence for the existence of two CAS isozymes. One of these isozymes was cloned and expressed in E. coli. The recombinant CAS isozyme was shown to catalyse the hydroxylation of (2S)-5-guanidino-2-(2′-oxoazetidin-1′-yl)pentanoic acid, in addition to the production of clavaminic acid from dihydroclavaminic acid via proclavaminic acid, consistent with the trifunctional role proposed for clavaminic acid synthase in the biosynthesis of clavulanic acid. A preliminary purification and characterisation of recombinant isozyme is reported. © 1994.

Publication status:: Published

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APA Style

Lawlor, E., Elson, S., Holland, S., Cassels, R., Hodgson, J., Lloyd, M., Baldwin, J., & Schofield, C. (1994). EXPRESSION IN ESCHERICHIA-COLI OF A CLAVAMINIC ACID SYNTHASE ISOZYME - A TRIFUNCTIONAL OXYGENASE INVOLVED IN CLAVULANIC ACID BIOSYNTHESIS. TETRAHEDRON, 50(29), 8737–8748.

MLA Style

Lawlor, E., et al. “EXPRESSION IN ESCHERICHIA-COLI OF A CLAVAMINIC ACID SYNTHASE ISOZYME - A TRIFUNCTIONAL OXYGENASE INVOLVED IN CLAVULANIC ACID BIOSYNTHESIS.” TETRAHEDRON, vol. 50, no. 29, 1994, pp. 8737–48.

Chicago Style

Lawlor, E, S Elson, S Holland, R Cassels, J Hodgson, M Lloyd, J Baldwin, and C Schofield. 1994. “EXPRESSION IN ESCHERICHIA-COLI OF A CLAVAMINIC ACID SYNTHASE ISOZYME - A TRIFUNCTIONAL OXYGENASE INVOLVED IN CLAVULANIC ACID BIOSYNTHESIS.” TETRAHEDRON 50 (29): 8737–48.
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Publisher copy:: 10.1016/S0040-4020(01)85347-3

Authors

+ Lawlor, E More by this author

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+ Elson, S More by this author

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+ Holland, S More by this author

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+ Cassels, R More by this author

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+ Hodgson, J More by this author

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Journal:: TETRAHEDRON More from this journal
Volume:: 50
Issue:: 29
Pages:: 8737-8748
Publication date:: 1994-07-18
DOI:: 10.1016/S0040-4020(01)85347-3
ISSN:: 0040-4020

Language:: English
Pubs id:: pubs:44475
UUID:: uuid:1fda5b84-5061-42d0-8512-5252308eb117
Local pid:: pubs:44475
Source identifiers:: 44475
Deposit date:: 2012-12-19

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Copyright date:: 1994

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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