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EXPRESSION IN ESCHERICHIA-COLI OF A CLAVAMINIC ACID SYNTHASE ISOZYME - A TRIFUNCTIONAL OXYGENASE INVOLVED IN CLAVULANIC ACID BIOSYNTHESIS

Abstract:

Purification and sequencing of clavaminic acid synthase (CAS) activities from S. clavuligerus SC2 provided evidence for the existence of two CAS isozymes. One of these isozymes was cloned and expressed in E. coli. The recombinant CAS isozyme was shown to catalyse the hydroxylation of (2S)-5-guanidino-2-(2′-oxoazetidin-1′-yl)pentanoic acid, in addition to the production of clavaminic acid from dihydroclavaminic acid via proclavaminic acid, consistent with the trifunctional role proposed for cl...

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Publication status:
Published

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Authors


HOLLAND, S More by this author
CASSELS, R More by this author
HODGSON, J More by this author
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Journal:
TETRAHEDRON
Volume:
50
Issue:
29
Pages:
8737-8748
Publication date:
1994-07-18
DOI:
ISSN:
0040-4020
URN:
uuid:1fda5b84-5061-42d0-8512-5252308eb117
Source identifiers:
44475
Local pid:
pubs:44475
Language:
English

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