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Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins

Abstract:

Structural, biochemical and biophysical studies of eukaryotic soluble and membrane proteins require their production in milligram quantities. Although large-scale protein expression strategies based on transient or stable transfection of mammalian cells are well established, they are associated with high consumable costs, limited transfection efficiency or long and tedious selection of clonal cell lines. Lentiviral transduction is an efficient method for the delivery of transgenes to mammalia...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Accepted Manuscript

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Publisher copy:
10.1038/s41596-018-0075-9

Authors


More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM
Subgroup:
Human Genetics Wt Centre
ORCID:
0000-0002-4196-4813
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM
Subgroup:
Human Genetics Wt Centre
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM
Subgroup:
Human Genetics Wt Centre
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM
Subgroup:
Human Genetics Wt Centre
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM
Subgroup:
Human Genetics Wt Centre
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Publisher:
Springer Nature Publisher's website
Journal:
Nature Protocols Journal website
Volume:
13
Pages:
2991–3017
Publication date:
2018-11-19
Acceptance date:
2018-11-01
DOI:
EISSN:
1750-2799
ISSN:
1754-2189
Pubs id:
pubs:945512
URN:
uri:1f9abeb7-4d33-4e30-aee6-6b72372260b6
UUID:
uuid:1f9abeb7-4d33-4e30-aee6-6b72372260b6
Local pid:
pubs:945512
Language:
English

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