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Live-cell studies of p300/CBP histone acetyltransferase activity and inhibition.

Abstract:

Histone acetyltransferase enzymes (HATs) are important therapeutic targets, but there are few cell-based assays available for evaluating the pharmacodynamics of HAT inhibitors. Here we present the application of a FRET-based reporter, Histac, in live-cell studies of p300/CBP HAT inhibition, by both genetic and pharmacologic disruption. shRNA knockdown of p300/CBP led to increased Histac FRET, thus suggesting a role for p300/CBP in the acetylation of the histone H4 tail. Additionally, we descr...

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Publication status:
Published

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Publisher copy:
10.1002/cbic.201200381

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Journal:
Chembiochem : a European journal of chemical biology
Volume:
13
Issue:
14
Pages:
2113-2121
Publication date:
2012-09-05
DOI:
EISSN:
1439-7633
ISSN:
1439-4227
URN:
uuid:1e7c4469-8afc-40cb-b2f8-b333b2f82a92
Source identifiers:
354704
Local pid:
pubs:354704

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