Journal article : Comment
Improving transient protein expression in agroinfiltrated Nicotiana benthamiana
- Abstract:
- Summary: Agroinfiltration of Nicotiana benthamiana is routinely used in plant science and molecular pharming to transiently express proteins of interest. Here, we discuss four phenomena that should be avoided to improve transient expression. Immune responses can be avoided by depleting immune receptors and employing pathogen‐derived effectors; transcript degradation by using silencing inhibitors or RNA interference machinery mutants; endoplasmic reticulum stress by co‐expressing chaperones; and protein degradation can be avoided with subcellular targeting, protease mutants and co‐expressing protease inhibitors. We summarise the reported increased yields for various recombinant proteins achieved with these approaches and highlight remaining challenges to further improve the efficiency of this versatile protein expression platform.
- Publication status:
- Published
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- Files:
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(Preview, Version of Record, Version of record, pdf, 439.8KB, Terms of use)
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- Publisher copy:
- 10.1111/nph.19894
Authors
- Publisher:
- Wiley
- Journal:
- New Phytologist More from this journal
- Publication date:
- 2024-06-07
- Acceptance date:
- 2024-05-02
- DOI:
- EISSN:
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1469-8137
- ISSN:
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1469-8137 and 0028-646X
- Language:
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English
- Keywords:
- Subtype:
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Comment
- Source identifiers:
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2027081
- Deposit date:
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2024-06-08
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