Journal article icon

Journal article

Diffusion of lipids and GPI-anchored proteins in actin-free plasma membrane vesicles measured by STED-FCS.

Abstract:

Diffusion and interaction dynamics of molecules at the plasma membrane play an important role in cellular signalling, and they are suggested to be strongly associated with the actin cytoskeleton. Here, we utilise super-resolution STED microscopy combined with fluorescence correlation spectroscopy (STED-FCS) to access and compare the diffusion characteristics of fluorescent lipid analogues and GPI-anchored proteins (GPI-APs) in the live cell plasma membrane and in actin cytoskeleton-free cell-...

Expand abstract
Publication status:
Published
Peer review status:
Peer reviewed

Actions


Access Document


Files:
Publisher copy:
10.1091/mbc.E16-07-0536

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
Weatherall Insti. of Molecular Medicine
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
Weatherall Insti. of Molecular Medicine
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
Weatherall Insti. of Molecular Medicine
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
Weatherall Insti. of Molecular Medicine
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
Weatherall Insti. of Molecular Medicine
Role:
Author
More from this funder
Name:
European Commission
Funding agency for:
Sezgin, E
Grant:
Marie Skłodowska-Curie Intra-European Fellowships (MEMBRANE DYNAMICS-627088
More from this funder
Name:
EMBO
Funding agency for:
Sezgin, E
Grant:
Marie Skłodowska-Curie Intra-European Fellowships (MEMBRANE DYNAMICS-627088
3024
More from this funder
Name:
Scientific and Technological Research Council of Turkey
Grant:
TUBITAK, grant number 114Z192
More from this funder
Name:
Wellcome Trust
Grant:
104924/14/Z/14
More from this funder
Name:
Biotechnology and Biological Sciences Research Council
Grant:
MR/K01577X/1
Publisher:
American Society for Cell Biology
Journal:
Molecular Biology of the Cell More from this journal
Volume:
28
Issue:
11
Pages:
1507-1518
Publication date:
2017-04-12
Acceptance date:
2017-04-04
DOI:
EISSN:
1939-4586
ISSN:
1059-1524
Language:
English
Keywords:
Pubs id:
pubs:691167
UUID:
uuid:1e49d975-0ca8-44e6-9923-f076adfd8b31
Local pid:
pubs:691167
Source identifiers:
691167
Deposit date:
2017-05-11

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP