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Diffusion of lipids and GPI-anchored proteins in actin-free plasma membrane vesicles measured by STED-FCS.

Abstract:

Diffusion and interaction dynamics of molecules at the plasma membrane play an important role in cellular signalling, and they are suggested to be strongly associated with the actin cytoskeleton. Here, we utilise super-resolution STED microscopy combined with fluorescence correlation spectroscopy (STED-FCS) to access and compare the diffusion characteristics of fluorescent lipid analogues and GPI-anchored proteins (GPI-APs) in the live cell plasma membrane and in actin cytoskeleton-free cell-...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1091/mbc.E16-07-0536

Authors


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Institution:
University of Oxford
Department:
Oxford, MSD, RDM, Weatherall Insti. of Molecular Medicine
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, RDM, Weatherall Insti. of Molecular Medicine
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, RDM, Weatherall Insti. of Molecular Medicine
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, RDM, Weatherall Insti. of Molecular Medicine
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, RDM, Weatherall Insti. of Molecular Medicine
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Funding agency for:
Sezgin, E
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Funding agency for:
Sezgin, E
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Grant:
MC_UU_12010/unit programmes G0902418 and MC_UU_12025, MR/K01577X/1
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Publisher:
American Society for Cell Biology Publisher's website
Journal:
Molecular Biology of the Cell Journal website
Volume:
28
Issue:
11
Pages:
1507-1518
Publication date:
2017-04-12
Acceptance date:
2017-04-04
DOI:
EISSN:
1939-4586
ISSN:
1059-1524
Pubs id:
pubs:691167
URN:
uri:1e49d975-0ca8-44e6-9923-f076adfd8b31
UUID:
uuid:1e49d975-0ca8-44e6-9923-f076adfd8b31
Local pid:
pubs:691167

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