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Probing the orientational distribution of dyes in membranes through multiphoton microscopy.

Abstract:

Numerous dyes are available or under development for probing the structural and functional properties of biological membranes. Exogenous chromophores adopt a range of orientations when bound to membranes, which have a drastic effect on their biophysical behavior. Here, we present a method that employs optical anisotropy data from three polarization-imaging techniques to establish the distribution of orientations adopted by molecules in monolayers and bilayers. The resulting probability densit...

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Publication status:
Published

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Publisher copy:
10.1016/j.bpj.2012.08.003

Authors


Corbett, AD More by this author
Boczarow, I More by this author
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Journal:
Biophysical journal
Volume:
103
Issue:
5
Pages:
907-917
Publication date:
2012-09-05
DOI:
EISSN:
1542-0086
ISSN:
0006-3495
URN:
uuid:1d415f03-0c41-48cd-ab92-9ac991580d15
Source identifiers:
350833
Local pid:
pubs:350833

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