Interleukin-6 and interleukin-11 support human osteoclast formation by a RANKL-independent mechanism.

Journal article

Interleukin-6 (IL-6) and interleukin-11 (IL-11) are known to influence osteoclast formation and bone resorption. In order to determine whether IL-6 and IL-11 could independently support human osteoclast formation, these factors were added to cultures of human peripheral blood mononuclear cells of the monocyte (CD14(+)) fraction in the presence of macrophage colony-stimulating factor (M-CSF). Under these conditions, IL-6 and IL-11 induced the formation of multinucleated cells which were positive for TRAP, VNR, and calcitonin receptor and capable of lacunar resorption. Osteoclastogenesis induced by IL-6 and IL-11 was inhibited by the addition of an anti-gp130 antibody but not by osteoprotegerin. These results indicate that IL-6 and IL-11, which are thought to play a role in several osteolytic bone disorders, are directly capable of inducing osteoclast formation by a RANKL-independent mechanism.

Authors: Kudo, O; Sabokbar, A; Pocock, A; Itonaga, I; Fujikawa, Y

• Publication status: Published
• Journal: Bone
• Volume: 32
• Issue: 1
• Pages: 1-7
• Publication date: 2003-01-01
• DOI: 10.1016/s8756-3282(02)00915-8
• EISSN: 1873-2763
• ISSN: 8756-3282
• Language: English
• Keywords: Carrier Proteins; Humans; Membrane Glycoproteins; Receptor Activator of Nuclear Factor-kappa B; Isoenzymes; Recombinant Proteins; Middle Aged; Female; Male; Osteoclasts; Interleukin-6; Interleukin-11; RANK Ligand; Acid Phosphatase; Cells, Cultured; Adult