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Quantitation of RNA polymerase II and its transcription factors in an HeLa cell: little soluble holoenzyme but significant amounts of polymerases attached to the nuclear substructure.

Abstract:

Various complexes that contain the core subunits of RNA polymerase II associated with different transcription factors have been isolated from eukaryotes; their precise molecular constitution depends on the purification procedure. We estimated the numbers of various components of such complexes in an HeLa cell by quantitative immunoblotting. The cells were lysed with saponin in a physiological buffer; approximately 140,000 unengaged polymerases (mainly of form IIA) were released. Only approxim...

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Publication status:
Published

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Authors


Roeder, RG More by this author
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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Journal:
Molecular and cellular biology
Volume:
19
Issue:
8
Pages:
5383-5392
Publication date:
1999-08-05
EISSN:
1098-5549
ISSN:
0270-7306
URN:
uuid:1c7b2240-c79a-49d6-823f-b4f3c538fbb3
Source identifiers:
9724
Local pid:
pubs:9724

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