Acridine orange fluorescence techniques as alternatives to traditional Giemsa staining for the diagnosis of malaria in developing countries.

Journal article

Traditional Giemsa-stained thick blood films were compared with 2 fluorescence microscopy techniques, acridine orange (AO) staining of thin blood films and the quantitative buffy coat (QBC) method, for the microscopical diagnosis of malaria. Of 200 samples examined, 141 were positive by Giemsa staining, 146 by AO and 137 by QBC. Overall sensitivities for the 2 fluorescence techniques compared to Giemsa staining were good: AO 97.9% and QBC 93.6%. However, with parasitaemias < 100/microL the...

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Authors: Lowe, B; Jeffa, N; New, L; Pedersen, C; Engbaek, K

• Publication status: Published
• Journal: Transactions of the Royal Society of Tropical Medicine and Hygiene
• Volume: 90
• Issue: 1
• Pages: 34-36
• Publication date: 1996-01-01
• DOI: 10.1016/s0035-9203(96)90470-8
• EISSN: 1878-3503
• ISSN: 0035-9203
• Language: English
• Keywords: Male; Staining and Labeling; Humans; Fluorescent Dyes; Plasmodium; Malaria; Azure Stains; Child; Acridine Orange; Child, Preschool; Animals; Female; Adult; Sensitivity and Specificity