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In vivo single-molecule imaging of bacterial DNA replication, transcription, and repair.

Abstract:

In vivo single-molecule experiments offer new perspectives on the behaviour of DNA binding proteins, from the molecular level to the length scale of whole bacterial cells. With technological advances in instrumentation and data analysis, fluorescence microscopy can detect single molecules in live cells, opening the doors to directly follow individual proteins binding to DNA in real time. In this review, we describe key technical considerations for implementing in vivo single-molecule fluoresc...

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Journal:
FEBS letters More from this journal
Volume:
588
Issue:
19
Pages:
3585-3594
Publication date:
2014-10-01
DOI:
EISSN:
1873-3468
ISSN:
0014-5793
Language:
English
Keywords:
Pubs id:
pubs:465881
UUID:
uuid:1bf72c00-b4d6-42b4-8363-907bf332aef4
Local pid:
pubs:465881
Source identifiers:
465881
Deposit date:
2014-06-27

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