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31P-CP-MAS NMR studies on TPP+ bound to the ion-coupled multidrug transport protein EmrE.

Abstract:

The binding of tetraphenylphosphonium (TPP+) to EmrE, a membrane-bound, 110 residue Escherichia coli multidrug transport protein, has been observed by 31P cross-polarisation-magic-angle spinning nuclear magnetic resonance spectroscopy (CP-MAS NMR). EmrE has been reconstituted into dimyristoyl phosphatidylcholine bilayers. CP-MAS could selectively distinguish binding of TPP+ to EmrE in the fluid membrane. A population of bound ligand appears shifted 4 ppm to lower frequency compared to free li...

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Publication status:
Published

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Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
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Journal:
FEBS letters
Volume:
480
Issue:
2-3
Pages:
127-131
Publication date:
2000-09-01
DOI:
EISSN:
1873-3468
ISSN:
0014-5793
Source identifiers:
100061

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