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Structure-activity correlations in pentachlorobenzene oxidation by engineered cytochrome P450cam.

Abstract:

We had reported engineering of the heme monooxygenase cytochrome P450cam from Pseudomonas putida with the F87W/Y96F/L244A/V247L mutations for the oxidation of pentachlorobenzene (PeCB), a recalcitrant environmental contaminant, to pentachlorophenol. In order to provide further insights into P450 structure, function and substrate recognition, we have determined the crystal structure of this 4-mutant without a substrate and its complex with PeCB. PeCB is bound face-on to the heme, with a weak F...

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Publication status:
Published

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Publisher copy:
10.1093/protein/gzm028

Authors


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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Inorganic Chemistry
Role:
Author
Journal:
Protein engineering, design and selection : PEDS More from this journal
Volume:
20
Issue:
10
Pages:
473-480
Publication date:
2007-10-01
DOI:
EISSN:
1741-0134
ISSN:
1741-0126
Language:
English
Keywords:
Pubs id:
pubs:40580
UUID:
uuid:1bab8039-194c-4978-9aa1-399d2850c814
Local pid:
pubs:40580
Source identifiers:
40580
Deposit date:
2012-12-19

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