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The effect of topology on the structure and free energy landscape of DNA kissing complexes.

Abstract:
We use a recently developed coarse-grained model for DNA to study kissing complexes formed by hybridization of complementary hairpin loops. The binding of the loops is topologically constrained because their linking number must remain constant. By studying systems with linking numbers -1, 0, or 1 we show that the average number of interstrand base pairs is larger when the topology is more favourable for the right-handed wrapping of strands around each other. The thermodynamic stability of the kissing complex also decreases when the linking number changes from -1 to 0 to 1. The structures of the kissing complexes typically involve two intermolecular helices that coaxially stack with the hairpin stems at a parallel four-way junction.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1063/1.4722203

Authors


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Institution:
University of Oxford
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Physics
Sub department:
Theoretical Physics
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Physics
Sub department:
Theoretical Physics
Role:
Author


Publisher:
American Institute of Physics
Journal:
Journal of chemical physics More from this journal
Volume:
136
Issue:
21
Pages:
215102
Publication date:
2012-06-01
DOI:
EISSN:
1089-7690
ISSN:
0021-9606


Language:
English
Keywords:
Pubs id:
pubs:320486
UUID:
uuid:1ba5018f-0375-4a2d-a24a-7faaef3faa87
Local pid:
pubs:320486
Source identifiers:
320486
Deposit date:
2013-03-20

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