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Mass spectrometry of ribosomes and ribosomal subunits.

Abstract:

Nanoflow electrospray ionization has been used to introduce intact Escherichia coli ribosomes into the ion source of a mass spectrometer. Mass spectra of remarkable quality result from a partial, but selective, dissociation of the particles within the mass spectrometer. Peaks in the spectra have been assigned to individual ribosomal proteins and to noncovalent complexes of up to five component proteins. The pattern of dissociation correlates strongly with predicted features of ribosomal prote...

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Publication status:
Published

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Publisher copy:
10.1073/pnas.95.13.7391
Journal:
Proceedings of the National Academy of Sciences of the United States of America More from this journal
Volume:
95
Issue:
13
Pages:
7391-7395
Publication date:
1998-06-01
DOI:
EISSN:
1091-6490
ISSN:
0027-8424
Language:
English
Keywords:
Pubs id:
pubs:59354
UUID:
uuid:1b9c5590-3063-4da3-8c41-7c6c26b2b2ac
Local pid:
pubs:59354
Source identifiers:
59354
Deposit date:
2012-12-19

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