In stimulated emission depletion (STED) nanoscopy the wavelength of the STED beam is usually tuned towards the red tail of the emission maximum of the fluorophore. Shifting the STED wavelength closer to the emission peak, i.e. towards the blue region, favorably increases the stimulated emission cross-section. However, this blue-shifting also increases the probability to excite fluorophores that have remained in their ground state, compromising the image contrast. Here we present a method to e...Expand abstract
- Publisher copy:
- Copyright date:
STED with wavelengths closer to the emission maximum.
If you are the owner of this record, you can report an update to it here: Report update to this record