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STED with wavelengths closer to the emission maximum.

Abstract:

In stimulated emission depletion (STED) nanoscopy the wavelength of the STED beam is usually tuned towards the red tail of the emission maximum of the fluorophore. Shifting the STED wavelength closer to the emission peak, i.e. towards the blue region, favorably increases the stimulated emission cross-section. However, this blue-shifting also increases the probability to excite fluorophores that have remained in their ground state, compromising the image contrast. Here we present a method to e...

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Publisher copy:
10.1364/oe.20.005225

Authors


Vicidomini, G More by this author
Moneron, G More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, RDM, Molecular Medicine
Rittweger, E More by this author
Publisher:
Optical Society of American (OSA)
Journal:
Optics express
Volume:
20
Issue:
5
Pages:
5225-5236
Publication date:
2012-02-05
DOI:
EISSN:
1094-4087
URN:
uuid:1b364a9d-415e-4137-be0f-74dbb0f2345d
Source identifiers:
316286
Local pid:
pubs:316286

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