Journal article

### STED with wavelengths closer to the emission maximum.

Abstract:

In stimulated emission depletion (STED) nanoscopy the wavelength of the STED beam is usually tuned towards the red tail of the emission maximum of the fluorophore. Shifting the STED wavelength closer to the emission peak, i.e. towards the blue region, favorably increases the stimulated emission cross-section. However, this blue-shifting also increases the probability to excite fluorophores that have remained in their ground state, compromising the image contrast. Here we present a method to e...

### Access Document

Publisher copy:
10.1364/oe.20.005225

### Authors

More by this author
Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
Weatherall Insti. of Molecular Medicine
Role:
Author
Publisher:
Optical Society of American (OSA)
Journal:
Optics express
Volume:
20
Issue:
5
Pages:
5225-5236
Publication date:
2012-02-01
DOI:
EISSN:
1094-4087
Source identifiers:
316286
Language:
English
Keywords:
Pubs id:
pubs:316286
UUID:
uuid:1b364a9d-415e-4137-be0f-74dbb0f2345d
Local pid:
pubs:316286
Deposit date:
2013-11-16