Kelp fly virus: a novel group of insect picorna-like viruses as defined by genome sequence analysis and a distinctive virion structure.

Hartley, C; Greenwood, DR; Gilbert, R; Masoumi, A; Gordon, K; Hanzlik, T; Fry, E; Stuart, D; Scotti, P

Journal article

Kelp fly virus: a novel group of insect picorna-like viruses as defined by genome sequence analysis and a distinctive virion structure.

Abstract:: The complete genomic sequence of kelp fly virus (KFV), originally isolated from the kelp fly, Chaetocoelopa sydneyensis, has been determined. Analyses of its genomic and structural organization and phylogeny show that it belongs to a hitherto undescribed group within the picorna-like virus superfamily. The single-stranded genomic RNA of KFV is 11,035 nucleotides in length and contains a single large open reading frame encoding a polypeptide of 3,436 amino acids with 5' and 3' untranslated regions of 384 and 343 nucleotides, respectively. The predicted amino acid sequence of the polypeptide shows that it has three regions. The N-terminal region contains sequences homologous to the baculoviral inhibitor of apoptosis repeat domain, an inhibitor of apoptosis commonly found in animals and in viruses with double-stranded DNA genomes. The second region contains at least two capsid proteins. The third region has three sequence motifs characteristic of replicase proteins of many plant and animal viruses, including a helicase, a 3C chymotrypsin-like protease, and an RNA-dependent RNA polymerase. Phylogenetic analysis of the replicase motifs shows that KFV forms a distinct and distant taxon within the picorna-like virus superfamily. Cryoelectron microscopy and image reconstruction of KFV to a resolution of 15 A reveals an icosahedral structure, with each of its 12 fivefold vertices forming a turret from the otherwise smooth surface of the 20-A-thick capsid. The architecture of the KFV capsid is unique among the members of the picornavirus superfamily for which structures have previously been determined.

Publication status:: Published

Actions

Email

Email this record

Send the bibliographic details of this record to your email address.

Your Email
Please enter the email address that the record information will be sent to.

-
Your message (optional)
Please add any additional information to be included within the email.
Share
Cite

Cite this record

APA Style

Hartley, C., Greenwood, D. R., Gilbert, R., Masoumi, A., Gordon, K., Hanzlik, T., Fry, E., Stuart, D., & Scotti, P. (2005). Kelp fly virus: a novel group of insect picorna-like viruses as defined by genome sequence analysis and a distinctive virion structure. Journal of Virology, 79(21), 13385–13398.

MLA Style

Hartley, C, et al. “Kelp Fly Virus: a Novel Group of Insect Picorna-like Viruses as Defined by Genome Sequence Analysis and a Distinctive Virion Structure.” Journal of Virology, vol. 79, no. 21, 2005, pp. 13385–98.

Chicago Style

Hartley, C, DR Greenwood, R Gilbert, et al. 2005. “Kelp Fly Virus: a Novel Group of Insect Picorna-like Viruses as Defined by Genome Sequence Analysis and a Distinctive Virion Structure.” Journal of Virology 79 (21): 13385–98.
Print