Drosophila Spd-2 recruits PCM to the sperm centriole, but is dispensable for centriole duplication.

Journal article

In C. elegans, genome-wide screens have identified just five essential centriole-duplication factors: SPD-2, ZYG-1, SAS-5, SAS-6, and SAS-4 [1-8]. These proteins are widely believed to comprise a conserved core duplication module [3, 9-14]. In worm embryos, SPD-2 is the most upstream component of this module, and it is also essential for pericentriolar material (PCM) recruitment to the centrioles [1, 4, 15, 16]. Here, we show that Drosophila Spd-2 (DSpd-2) is a component of both the centrioles and the PCM and has a role in recruiting PCM to the centrioles. DSpd-2 appears not, however, to be essential for centriole duplication in somatic cells. Moreover, PCM recruitment in DSpd-2 mutant somatic cells is only partially compromised, and mitosis appears unperturbed. In contrast, DSpd-2 is essential for proper PCM recruitment to the fertilizing sperm centriole, and hence for microtubule nucleation and pronuclear fusion. DSpd-2 therefore appears to have a particularly important role in recruiting PCM to the sperm centriole. We speculate that the SPD-2 family of proteins might only be absolutely essential for the recruitment of centriole duplication factors and PCM to the centriole(s) that enter the egg with the fertilizing sperm.

Authors: Dix, C; Raff, J

• Publication status: Published
• Journal: Current biology : CB
• Volume: 17
• Issue: 20
• Pages: 1759-1764
• Publication date: 2007-10-01
• DOI: 10.1016/j.cub.2007.08.065
• EISSN: 1879-0445
• ISSN: 0960-9822
• Language: English
• Keywords: Drosophila; Mitosis; Fertilization; Spermatozoa; Meiosis; Male; Centrioles; Animals; Caenorhabditis elegans; Caenorhabditis elegans Proteins; Drosophila Proteins