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Reconstruction of complexes of histone and superhelical nuclear DNA.

Abstract:

When HeLa cells are lysed in solutions containing a non-ionic detergent and 2 M-NaCl, structures are released that retain many of the morphological features of nuclei. These nucleoids contain all the nuclear RNA and DNA but few of the proteins characteristic of chromatin. Their DNA is supercoiled and so intact. Using a simple and rapid procedure we have reconstructed nucleohistone complexes from nucleoids and the 'core' histones without breaking the DNA. We have probed the integrity and struc...

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Publication status:
Published

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Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
Journal:
Journal of cell science
Volume:
50
Issue:
AUG
Pages:
209-224
Publication date:
1981-08-01
EISSN:
1477-9137
ISSN:
0021-9533
Source identifiers:
4665
Language:
English
Keywords:
Pubs id:
pubs:4665
UUID:
uuid:1ac6370b-6106-4ee4-b985-c43eb44a38f5
Local pid:
pubs:4665
Deposit date:
2012-12-19

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