Journal article
Reconstruction of complexes of histone and superhelical nuclear DNA.
- Abstract:
-
When HeLa cells are lysed in solutions containing a non-ionic detergent and 2 M-NaCl, structures are released that retain many of the morphological features of nuclei. These nucleoids contain all the nuclear RNA and DNA but few of the proteins characteristic of chromatin. Their DNA is supercoiled and so intact. Using a simple and rapid procedure we have reconstructed nucleohistone complexes from nucleoids and the 'core' histones without breaking the DNA. We have probed the integrity and struc...
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- Publication status:
- Published
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Bibliographic Details
- Journal:
- Journal of cell science
- Volume:
- 50
- Issue:
- AUG
- Pages:
- 209-224
- Publication date:
- 1981-08-01
- EISSN:
-
1477-9137
- ISSN:
-
0021-9533
- Source identifiers:
-
4665
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:4665
- UUID:
-
uuid:1ac6370b-6106-4ee4-b985-c43eb44a38f5
- Local pid:
- pubs:4665
- Deposit date:
- 2012-12-19
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- Copyright date:
- 1981
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