Journal article
High-resolution mass spectrometry of small molecules bound to membrane proteins
- Abstract:
- Small molecules are known to stabilize membrane proteins and to modulate their function and oligomeric state, but such interactions are often hard to precisely define. Here we develop and apply a high-resolution, Orbitrap mass spectrometry–based method for analyzing intact membrane protein–ligand complexes. Using this platform, we resolve the complexity of multiple binding events, quantify small molecule binding and reveal selectivity for endogenous lipids that differ only in acyl chain length.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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Access Document
- Files:
-
-
(Preview, Accepted manuscript, pdf, 1.2MB, Terms of use)
-
- Publisher copy:
- 10.1038/nmeth.3771
Authors
Bibliographic Details
- Publisher:
- Springer Nature
- Journal:
- Nature methods More from this journal
- Volume:
- 13
- Issue:
- 4
- Pages:
- 333-336
- Publication date:
- 2016-04-01
- Acceptance date:
- 2016-01-13
- DOI:
- EISSN:
-
1548-7105
- ISSN:
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1548-7091
- Pmid:
-
26901650
Item Description
- Language:
-
English
- Keywords:
- Pubs id:
-
pubs:605932
- UUID:
-
uuid:1a81bf2a-17d1-4bb0-bc7b-7cdb1345b9dc
- Local pid:
-
pubs:605932
- Source identifiers:
-
605932
- Deposit date:
-
2016-12-09
Terms of use
- Copyright holder:
- Springer Nature Limited
- Copyright date:
- 2016
- Notes:
- © 2018 Springer Nature Limited. This is the accepted manuscript version of the article. The final version is available online from Springer Nature at: 10.1038/nmeth.3771
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