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Production of monoclonal antibodies to lactate dehydrogenase (LDH) isoenzymes for immunohistochemical study on fixed tissue section.

Abstract:

Purified lactate dehydrogenase (LDH) isoenzyme 1 (H or B subunits) and isoenzyme 5 (M or A subunits) were used to prepare monoclonal antibodies (MAb) suitable for immunohistochemical detection on formalin fixed paraffin-embedded tissue sections. In the initial fusions, screening of the antibodies was based on enzyme linked immunosorbent assay (ELISA) against the immunogens. None of the antibodies obtained was satisfactory. There were various problems related to specificity, crossreactivity, a...

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Publisher copy:
10.1007/bf01002483

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Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Experimental Medicine Division,
Role:
Author
Journal:
The Histochemical journal
Volume:
21
Issue:
11
Pages:
638-644
Publication date:
1989-11-05
DOI:
EISSN:
1573-6865
ISSN:
0018-2214
URN:
uuid:1a3a4241-0782-46d8-85e9-f1cc8c5de336
Source identifiers:
35216
Local pid:
pubs:35216

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