Journal article
Production of monoclonal antibodies to lactate dehydrogenase (LDH) isoenzymes for immunohistochemical study on fixed tissue section.
- Abstract:
-
Purified lactate dehydrogenase (LDH) isoenzyme 1 (H or B subunits) and isoenzyme 5 (M or A subunits) were used to prepare monoclonal antibodies (MAb) suitable for immunohistochemical detection on formalin fixed paraffin-embedded tissue sections. In the initial fusions, screening of the antibodies was based on enzyme linked immunosorbent assay (ELISA) against the immunogens. None of the antibodies obtained was satisfactory. There were various problems related to specificity, crossreactivity, a...
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- Publisher copy:
- 10.1007/bf01002483
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Bibliographic Details
- Journal:
- Histochemical journal
- Volume:
- 21
- Issue:
- 11
- Pages:
- 638-644
- Publication date:
- 1989-11-01
- DOI:
- EISSN:
-
1573-6865
- ISSN:
-
0018-2214
- Source identifiers:
-
35216
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:35216
- UUID:
-
uuid:1a3a4241-0782-46d8-85e9-f1cc8c5de336
- Local pid:
- pubs:35216
- Deposit date:
- 2012-12-20
Terms of use
- Copyright date:
- 1989
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