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Journal article

Analysis of conditions affecting auto-phosphorylation of human kinases during expression in bacteria.

Abstract:

Bacterial over-expression of kinases is often associated with high levels of auto-phosphorylation resulting in heterogeneous recombinant protein preparations or sometimes in insoluble protein. Here we present expression systems for nine kinases in Escherichia coli and, for the most heavily phosphorylated, the characterisation of factors affecting auto-phosphorylation. Experiments showed that the level of auto-phosphorylation was proportional to the rate of expression. Comparison of phosphoryl...

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Publication status:
Published

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Publisher copy:
10.1016/j.pep.2011.09.012

Authors


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Institution:
University of Oxford
Department:
Oxford, MSD, Oncology
Role:
Author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Structural Genomics Consortium
Role:
Author
Journal:
Protein expression and purification
Volume:
81
Issue:
1
Pages:
136-143
Publication date:
2012-01-05
DOI:
EISSN:
1096-0279
ISSN:
1046-5928
URN:
uuid:1a013e5a-8343-406f-a586-00824c2e733b
Source identifiers:
186649
Local pid:
pubs:186649

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