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Antiretroviral therapy blocks natural selection on protective and disease-susceptible HLA-B alleles in HIV-1 infection

Abstract:
MHC polymorphism is explained by natural selection driven by the MHC-dependent impact of certain infections, inflammatory conditions, autoimmune diseases, and cancers. However, examples of human disease driving this process are rare. We evaluated the impact of HIV-1 in altering HLA-I frequencies in KwaZulu-Natal, South Africa, and the influence of antiretroviral therapy (ART) on this process. In a historical mother-child cohort in the pre-ART era (1998–2005), HIV-1 survival and vertical transmission were both strongly HLA-B dependent: “disease-susceptible” HLA-B alleles (HLA-B*18/B*45:01/B*58:02) increased adult AIDS progression and vertical transmission (OR 1.6, P = 0.01), whereas “protective” HLA-B alleles (HLA-B*57/B*58:01/B*81:01) slowed AIDS progression, and decreased vertical transmission (OR 0.57, P = 0.002). By contrast, in contemporary antenatal KwaZulu-Natal cohorts in the ART era (2015–2025) the impact of HLA-B on HIV-1 disease outcome and vertical transmission is dramatically reduced. Using these and reported data, we constructed a model to estimate the impact of HIV-1 on HLA-B frequencies in KwaZulu-Natal, both in the prevailing setting of ART and in a hypothetical counterfactual scenario where ART was never rolled out. Over the 45-y period 1990–2035, in the absence of ART, the proportion of the population possessing any “protective” HLA-B allele was projected to increase from 23 to 42% (allele frequencies increasing from 0.12 to 0.24), and the proportion of the population possessing any “disease-susceptible” HLA-B allele was projected to decrease from 28 to 18% (allele frequencies declining from 0.15 to 0.092). The introduction of ART radically slows HLA-B frequency change. These data therefore demonstrate the potential for natural selection from an infectious disease to alter human population genetics within decades, and for the successful roll-out of therapy to halt this process.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1073/pnas.2502683123

Authors

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Institution:
University of Oxford
Division:
MSD
Department:
Paediatrics
Role:
Author
ORCID:
0000-0002-3708-694X
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Role:
Author
ORCID:
0000-0003-2748-1572
More by this author
Role:
Author
ORCID:
0009-0005-4694-8475


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Funder identifier:
https://ror.org/029chgv08
Grant:
WT104748MA
More from this funder
Funder identifier:
https://ror.org/01cwqze88
Grant:
U01AI168655
AI133673
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Funder identifier:
https://ror.org/03v6m3209
Grant:
75N91019D00024


Publisher:
National Academy of Sciences
Journal:
Proceedings of the National Academy of Sciences More from this journal
Volume:
123
Issue:
19
Article number:
e2502683123
Publication date:
2026-04-27
Acceptance date:
2026-03-18
DOI:
EISSN:
1091-6490
ISSN:
0027-8424


Language:
English
Keywords:
Pubs id:
2412143
Local pid:
pubs:2412143
Deposit date:
2026-04-28
ARK identifier:

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