Book section : Chapter

In vitro migration assays

Abstract:: The timely recruitment of innate and adaptive immune cells to sites of inflammation and repair is essential for host defense against pathogens and repair of damaged tissues. The development of bioassays such as in vitro chemotaxis assays played an important role in the original purification of chemoattractant cytokines including interleukin-1 and the CC and CXC chemokines. The earliest chemotaxis methods were based on the principle of the Boyden chamber, first described in 1962. In this chapter we give detailed protocols for more recent techniques that allow determination of macrophage chemotaxis in real time. These techniques have given new insights into the regulation of macrophage responses to chemotaxis in vitro and in vivo.

Publication status:: Published

Peer review status:: Peer reviewed

Actions

Email

Email this record

Send the bibliographic details of this record to your email address.

Your Email
Please enter the email address that the record information will be sent to.

-
Your message (optional)
Please add any additional information to be included within the email.
Cite

Cite this record

APA Style

Taylor, L., Recio, C., Greaves, D. R., & Iqbal, A. J. (2018). In vitro migration assays. In G. Rousselet (Ed.), Macrophages (pp. 197–214). Humana Press.

MLA Style

Taylor, L., et al. “In Vitro Migration Assays.” Macrophages, edited by G Rousselet, Humana Press, 2018, pp. 197–214.

Chicago Style

Taylor, L, C Recio, DR Greaves, and AJ Iqbal. 2018. “In Vitro Migration Assays.” In Macrophages, edited by G Rousselet, 197–214. Methods in Molecular Biology. Humana Press.
Share
Print

Access Document

Publisher copy:: 10.1007/978-1-4939-7837-3_19

Authors

+ Taylor, L More by this author

Institution:: University of Oxford
Division:: MSD
Department:: Pathology Dunn School
Role:: Author

+ Recio, C More by this author

Institution:: University of Oxford
Division:: MSD
Department:: Pathology Dunn School
Role:: Author

+ Greaves, DR More by this author

Institution:: University of Oxford
Division:: MSD
Department:: Pathology Dunn School
Role:: Author
ORCID:: 0000-0003-2856-9410

+ Iqbal, AJ More by this author

Role:: Author
ORCID:: 0000-0002-3224-3651

Contributors

Role:: Editor

+ British Heart Foundation More from this funder

Grant:: RG/15/10/31485

+ Edward Penley Abraham Research Fund More from this funder

Grant:: RF238

Publisher:: Humana Press
Host title:: Macrophages
Pages:: 197-214
Series:: Methods in Molecular Biology
Series number:: 1784
Publication date:: 2018-05-15
DOI:: 10.1007/978-1-4939-7837-3_19
EISSN:: 1940-6029
ISSN:: 1064-3745
Pmid:: 29761401
EISBN:: 978-1-4939-7837-3
ISBN:: 978-1-4939-7836-6

Language:: English
Keywords:: migration

chemotaxis

chemokines

macrophages

inflammation
Subtype:: Chapter
Pubs id:: pubs:853098
UUID:: uuid:19a2a297-a1ab-4c26-a7da-3c68da5d810a
Local pid:: pubs:853098
Source identifiers:: 853098
Deposit date:: 2018-07-04

Terms of use

Copyright holder:: Springer Science+Business Media, LLC
Copyright date:: 2018
Rights statement:: © 2018 Springer Science+Business Media, LLC, part of Springer Nature

Licence:: Terms and Conditions of Use for Oxford University Research Archive

Views and Downloads

About views and downloads

If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP