Journal article
Ligation of double-stranded and single-stranded [Oligo(dT)] DNA by vaccinia virus DNA ligase
- Abstract:
- Vaccinia virus DNA ligase has been expressed in Escherichia coli, purified, and biochemically characterized. The enzyme ligates double-stranded (ds) DNA substrates with either cohesive or blunt-end termini and the latter reaction is stimulated by PEG. Vaccinia virus DNA ligase can also ligate oligo(dT) when annealed to either a poly(dA) or a poly(rA) backbone and, remarkably, free oligo(dT). This ligation of a single-stranded (ss) substrate is unique among eukaryotic DNA ligases. The enzyme requires high ATP concentrations with a Km for the overall ligation of a ssDNA substrate of 0.8 mM. The salt, divalent cation, temperature, and pH requirements of the enzyme for the optimal ligation of ss and ds substrate are described.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
Actions
Access Document
- Files:
-
-
(Preview, Version of record, pdf, 191.0KB, Terms of use)
-
- Publisher copy:
- 10.1006/viro.1996.0358
Authors
- Publisher:
- Elsevier
- Journal:
- Virology More from this journal
- Volume:
- 221
- Issue:
- 1
- Pages:
- 120–129
- Publication date:
- 1996-07-01
- Edition:
- Publisher's version
- DOI:
- ISSN:
-
0042-6822
- Language:
-
English
- Subjects:
- UUID:
-
uuid:196eaad0-e524-421a-855b-eb3781d880df
- Local pid:
-
ora:10722
- Deposit date:
-
2015-03-25
- ARK identifier:
Terms of use
- Copyright holder:
- Academic Press, Inc
- Copyright date:
- 1996
- Notes:
- Copyright © 1996 Academic Press. All rights reserved. Re-use of this article is permitted in accordance with the Terms and Conditions set out at http://www.elsevier.com/open-access/userlicense/1.0/
- Licence:
- Other
If you are the owner of this record, you can report an update to it here: Report update to this record