Cell interactions in the immune response in vitro. VI. Mediation by T cell surface monomeric IgM.

Journal article

The nature of the specific mediator of T/B lymphocyte cooperation was investigated by using biochemical and in vitro culture methods in parallel. By surface radioiodination of activated thymus cells it was found that the T cell component of cell interaction was an immunoglobulin which possessed a mobility on polyacrylamide gel electrophoresis consistent with a molecular weight of approximately 180,000. This molecule comprised of two polypeptide chains, with the electrophoretic mobility of light and μ chains. This monomeric IgM molecule had specificity for the activating antigen, and like the mediator of cell interaction, bound cytophilically to macrophages, but not to T or B lymphocytes. These studies extend previous work in which the mediator of cell cooperation was found, by the use of antisera to contain antigen, and κ and μ chain determinants. The binding of the T cell surface IgM to macrophages supports the concept that the final step of cell cooperation, the immunization of B cells occurs at the surface of macrophages, occurs via a lattice of antigenic determinants created by cytophilic T cell carrier antibody. © 1973.

Authors: Feldmann, M; Cone, R; Marchalonis, J

• Journal: Cellular immunology
• Volume: 9
• Issue: 1
• Pages: 1-11
• Publication date: 1973-10-01
• DOI: 10.1016/0008-8749(73)90162-7
• EISSN: 1090-2163
• ISSN: 0008-8749
• Language: English
• Keywords: gamma-Globulins; Radiation Chimera; Mice; Ascitic Fluid; Dinitrophenols; Immune Sera; Hemolytic Plaque Technique; Epitopes; Protein Binding; B-Lymphocytes; Antibody-Producing Cells; Hemocyanin; Immunoglobulin M; Electrophoresis, Polyacrylamide Gel; Iodine Radioisotopes; Spleen; Chickens; Animals; Macrophages; T-Lymphocytes; Rabbits; Peptides; Cell Membrane; Salmonella; Flagella; Cells, Cultured