- Abstract:
-
The interpretation of genome sequences requires reliable and standardized methods to assess protein function at high throughput. Here we describe a fast and reliable pipeline to study protein function in mammalian cells based on protein tagging in bacterial artificial chromosomes (BACs). The large size of the BAC transgenes ensures the presence of most, if not all, regulatory elements and results in expression that closely matches that of the endogenous gene. We show that BAC transgenes can b...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1038/nmeth.1199
-
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- Journal:
- Nature methods
- Volume:
- 5
- Issue:
- 5
- Pages:
- 409-415
- Publication date:
- 2008-05-05
- DOI:
- EISSN:
-
1548-7105
- ISSN:
-
1548-7091
- URN:
-
uuid:19433e36-e5b5-4a80-9d4e-d0b912701619
- Source identifiers:
-
98342
- Local pid:
- pubs:98342
- Copyright date:
- 2008
Journal article
BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals.
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