Immunologic characterization of monoclonal antibodies that modulate human IgE binding to the major birch pollen allergen Bet v 1.

Lebecque, S; Dolecek, C; Laffer, S; Visco, V; Denépoux, S; Pin, J; Guret, C; Boltz-Nitulescu, G; Weyer, A; Valenta, R

Journal article

Immunologic characterization of monoclonal antibodies that modulate human IgE binding to the major birch pollen allergen Bet v 1.

Abstract:: BACKGROUND: Bet v 1 and homologous proteins represent major allergens for almost 95% of patients allergic to tree pollen and approximately 70% of those allergic to fruits and vegetables. As yet, no continuous (sequential) IgE epitopes have been determined for Bet v 1, and evidence has accumulated that Bet v 1 IgE epitopes belong to the conformational (discontinuous) type. OBJECTIVE: A panel of 85 mouse monoclonal anti-Bet v 1 antibodies was raised as a tool with which to study the interaction of human IgE antibodies with Bet v 1. METHODS: The epitopes of selected monoclonal antibodies (mAbs) were characterized by mapping with synthetic overlapping peptides and by cross-competition experiments. Cross-reactivity of Bet v 1-specific mAbs with tree and plant food allergens was investigated by Western blotting. The influence of Bet v 1-specific mAbs on the IgE-Bet v 1 interaction was studied by competition assays with immobilized purified recombinant Bet v 1 and by basophil histamine release experiments. RESULTS: Antibodies that increased the IgE binding to Bet v 1 up to fivefold could be defined, whereas others inhibited IgE binding to Bet v 1 up to 99% and competed with the Bet v 1-induced histamine release from patients' basophils. CONCLUSION: The activity of the enhancing antibodies is interpreted as a stabilization of Bet v 1 states/IgE epitopes, which are either more accessible for certain IgE antibodies or are recognized with higher affinity. Those mAbs that competed with the Bet v 1-IgE interaction, if humanized or produced as recombinant antibody fragments, might be considered as potential tools for local allergy therapy.

Publication status:: Published

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APA Style

Lebecque, S., Dolecek, C., Laffer, S., Visco, V., Denépoux, S., Pin, J., Guret, C., Boltz-Nitulescu, G., Weyer, A., & Valenta, R. (1997). Immunologic characterization of monoclonal antibodies that modulate human IgE binding to the major birch pollen allergen Bet v 1. Journal of Allergy and Clinical Immunology, 99(3), 374–384.

MLA Style

Lebecque, S, et al. “Immunologic Characterization of Monoclonal Antibodies That Modulate Human IgE Binding to the Major Birch Pollen Allergen Bet v 1.” Journal of Allergy and Clinical Immunology, vol. 99, no. 3, 1997, pp. 374–84.

Chicago Style

Lebecque, S, C Dolecek, S Laffer, et al. 1997. “Immunologic Characterization of Monoclonal Antibodies That Modulate Human IgE Binding to the Major Birch Pollen Allergen Bet v 1.” Journal of Allergy and Clinical Immunology 99 (3): 374–84.
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