Preprint
Enhanced muscle uptake of chemically optimized miR-23b antisense oligonucleotides as lead compounds for Myotonic Dystrophy type 1
- Abstract:
- Myotonic dystrophy type 1 (DM1) is a multisystemic disorder caused by CTG repeat expansions in DM1 protein kinase DMPK. Mutant transcripts containing expanded CUG repeats form ribonuclear foci that sequester muscleblind-like (MBNL) splicing regulator proteins, key regulators of RNA splicing and metabolism. This functional depletion leads to widespread mis-splicing and persistence of fetal transcript profiles, which underlie muscle weakness, myotonia, and muscle atrophy. In addition, miR-23b is upregulated in DM1 muscle and further represses MBNL1 translation, amplifying molecular defects. We developed chemically optimized microRNA (miRNA)-targeting antisense oligonucleotides (antimiRs) to inhibit miR-23b and restore functional MBNL1 levels. Using a multistep screening process, we evaluated antimiRs with varying sequences, lengths, chemical modifications, and lipid conjugations. A key optimization was a 3′ -oleic acid conjugation combined with specific chemical modifications, which enhanced muscle uptake and efficacy. Lead candidates showed strong activity in preclinical models (human skeletal actin [HSA]LR and DMSXL mice and human myoblasts), increasing MBNL1 levels, correcting mis-splicing, improving muscle strength, and reducing myotonia. They also exhibited efficient biodistribution to skeletal muscle, a critical DM1-affected tissue. In vitro toxicology indicated a favorable safety profile with minimal immune or renal toxicity. The antimiR mechanism was conserved in rat and pig fibroblasts. Overall, two lead antimiRs emerged as promising therapeutic candidates for DM1, with improved pharmacokinetics, tissue targeting, and safety, supporting the potential of microRNA-based approaches to correct key molecular defects in this disorder.
- Publication status:
- Published
- Peer review status:
- Not peer reviewed
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(Preview, Pre-print, pdf, 1.9MB, Terms of use)
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- Preprint server copy:
- 10.1101/2025.07.30.667638
Authors
+ Generalitat Valenciana
More from this funder
- Funder identifier:
- https://ror.org/0097mvx21
- Grant:
- PROMETEO/2020/081
- CIPROM/2023/22
- FDEGENT/2020/011
+ Instituto de Salud Carlos III
More from this funder
- Funder identifier:
- https://ror.org/00ca2c886
- Grant:
- DTS19/0128
- PI21/00557
+ Centre for Industrial Technological Development
More from this funder
- Funder identifier:
- https://ror.org/0124x7055
- Grant:
- SNEO-20201136
+ Instituto Valenciano de Competitividad Empresarial
More from this funder
- Funder identifier:
- https://ror.org/01svxr124
- Grant:
- IMIDTA/ 2021/65
- Preprint server:
- bioRxiv
- Publication date:
- 2026-02-02
- DOI:
- Language:
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English
- Keywords:
- Pubs id:
-
2277237
- Local pid:
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pubs:2277237
- Deposit date:
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2026-03-06
- ARK identifier:
Terms of use
- Copyright holder:
- Gonzalez-Martinez et al.
- Copyright date:
- 2026
- Rights statement:
- The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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