Journal article
SIMcheck: a toolbox for successful super-resolution structured illumination microscopy
- Abstract:
- Three-dimensional structured illumination microscopy (3D-SIM) is a versatile and accessible method for super-resolution fluorescence imaging, but generating high-quality data is challenging, particularly for non-specialist users. We present SIMcheck, a suite of ImageJ plugins enabling users to identify and avoid common problems with 3D-SIM data, and assess resolution and data quality through objective control parameters. Additionally, SIMcheck provides advanced calibration tools and utilities for common image processing tasks. This open-source software is applicable to all commercial and custom platforms, and will promote routine application of super-resolution SIM imaging in cell biology.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Version of record, pdf, 105.1KB, Terms of use)
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(Preview, Version of record, pdf, 1.8MB, Terms of use)
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- Publisher copy:
- 10.1038/srep15915
Authors
- Publisher:
- Nature Publishing Group
- Journal:
- Scientific Reports More from this journal
- Volume:
- 3
- Publication date:
- 2015-03-03
- Acceptance date:
- 2015-03-03
- DOI:
- ISSN:
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2045-2322
- Pmid:
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26525406
- Language:
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English
- Keywords:
- Pubs id:
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pubs:572637
- UUID:
-
uuid:180c0af9-c3a6-41a6-8812-447ac434e967
- Local pid:
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pubs:572637
- Deposit date:
-
2016-10-07
Terms of use
- Copyright holder:
- Graeme Ball et al
- Copyright date:
- 2015
- Notes:
-
This work is licensed under a Creative Commons Attribution 4.0 International License. The images
or other third party material in this article are included in the article’s Creative Commons license,
unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license,
users will need to obtain permission from the license holder to reproduce the material. To view a copy of this
license, visit http://creativecommons.org/licenses/by/4.0/
- Licence:
- CC Attribution (CC BY)
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