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Catecholamine interference with enzymatic determination of nonesterified fatty acids in two commercially available test kits.

Abstract:

We present evidence that catecholamines, which are commonly used to stimulate lipolysis in adipose tissue in vitro, interfere with the enzymatic determination of non-esterified fatty acid (NEFA) in two commercially available kits. Measurement of a 100 mumol/L standard with the Wako "NEFA C" test kit was 60% inhibited by 100 mumol/L norepinephrine and was completely inhibited by 100 mumol/L isoproterenol or by 1 mmol/L norepinephrine or epinephrine. Measurement with the Boehringer Mannheim "Fr...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MSD, RDM, OCDEM
Role:
Author
Journal:
Clinical chemistry
Volume:
41
Issue:
3
Pages:
455-457
Publication date:
1995-03-05
EISSN:
1530-8561
ISSN:
0009-9147
URN:
uuid:17ccf696-1849-490f-ac46-2a761725d8d1
Source identifiers:
27420
Local pid:
pubs:27420

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