Ha-ras hypervariable alleles in myelodysplasia.

Journal article

The somatic mutation of one of the ras oncogenes is now considered to be a critical step in the pathogenesis of many tumours. Circumstantial evidence also suggests that some individuals may be genetically predisposed to malignancy and a general method used to analyse such disease susceptibility is the study of restriction fragment length polymorphisms (RFLPs) at particular loci. The Harvey ras (Ha-ras) locus includes a hypervariable region (HVR) which consists of a series of 28-base-pair (bp) tandem repeats 3' to the gene. This arrangement gives rise to alleles of a wide range of sizes, making such genetic analysis possible. A previous study reported that white blood cell DNA from cancer patients frequently showed allelic restriction fragments at the Ha-ras locus which were found only rarely in normal unaffected individuals, and it was concluded that the inheritance of such unusual alleles may be linked to a susceptibility to cancer. As this conclusion has major implications we sought to investigate whether this association could be confirmed in patients with myelodysplasia, a common haematological malignancy reported to have the highest frequency of rare alleles. The Ha-ras alleles were characterized in normal healthy individuals and compared with those found in patients with myelodysplasia (MDS). Our results, reported here, show that the distribution of Ha-ras alleles in myelodysplastic patients is not significantly different from that in normal individuals.

Authors: Thein, S; Oscier, D; Flint, J; Wainscoat, J

• Publication status: Published
• Journal: Nature
• Volume: 321
• Issue: 6065
• Pages: 84-85
• Publication date: 1986-01-01
• DOI: 10.1038/321084a0
• EISSN: 1476-4687
• ISSN: 0028-0836
• Language: English
• Keywords: Mutation; Leukocytes; Oncogenes; Humans; Reference Values; DNA Restriction Enzymes; Myelodysplastic Syndromes; Genetic Variation; Alleles; DNA