Journal article
Rapid chemical ligation of oligonucleotides by the Diels-Alder reaction.
- Abstract:
- Extremely fast and efficient Diels-Alder chemical ligation of furan and maleimide oligonucleotides has been carried out in aqueous buffer. It was possible to ligate three oligonucleotides simultaneously in a controlled manner with the aid of a complementary splint. The templated reactions proceeded within 1 min at room temperature whereas non-templated reactions were slow and incomplete. Rapid and clean methods of DNA ligation such as the one demonstrated here have potential uses in biology and nanotechnology.
- Publication status:
- Published
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- Publisher copy:
- 10.1039/c0ob00451k
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Authors
Bibliographic Details
- Journal:
- Organic and biomolecular chemistry
- Volume:
- 9
- Issue:
- 1
- Pages:
- 232-235
- Publication date:
- 2011-01-01
- DOI:
- EISSN:
-
1477-0539
- ISSN:
-
1477-0520
- Source identifiers:
-
399995
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:399995
- UUID:
-
uuid:179a8293-e89a-4517-8850-0e1c052c3fdf
- Local pid:
- pubs:399995
- Deposit date:
- 2013-11-16
Terms of use
- Copyright date:
- 2011
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