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Rapid chemical ligation of oligonucleotides by the Diels-Alder reaction.

Abstract:
Extremely fast and efficient Diels-Alder chemical ligation of furan and maleimide oligonucleotides has been carried out in aqueous buffer. It was possible to ligate three oligonucleotides simultaneously in a controlled manner with the aid of a complementary splint. The templated reactions proceeded within 1 min at room temperature whereas non-templated reactions were slow and incomplete. Rapid and clean methods of DNA ligation such as the one demonstrated here have potential uses in biology and nanotechnology.
Publication status:
Published

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Publisher copy:
10.1039/c0ob00451k

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Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Organic Chemistry
Role:
Author
Journal:
Organic and biomolecular chemistry
Volume:
9
Issue:
1
Pages:
232-235
Publication date:
2011-01-05
DOI:
EISSN:
1477-0539
ISSN:
1477-0520
URN:
uuid:179a8293-e89a-4517-8850-0e1c052c3fdf
Source identifiers:
399995
Local pid:
pubs:399995

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