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Is it time to revisit the scoring of slow wave (N3) sleep?

Abstract:

The use of a fixed electroencephalogram (EEG) amplitude threshold of 75 µV for labeling slow waves is a subject of ongoing discussion given EEG amplitude is known to vary with age and sex. This paper investigates the impact of this amplitude threshold on age- and sex-related trends in visually annotated slow wave sleep (SWS). Automated methods for labeling SWS using data-driven thresholds and amplitude- or frequency-based inputs are developed. Age- and sex-related trends in SWS derived from visual annotation and automated labeling are then compared across a cohort of 2913 participants from the Sleep Heart Health Study.

In the selected cohort, males exhibit an age-related decrease in visually annotated SWS, which is preserved when using automated labeling. In contrast, females exhibit a mild age-related increase in visually annotated and amplitude-labeled SWS, but an age-related decrease in frequency-labeled SWS. Furthermore, using frequency-labeled SWS results in a reduction in SWS in females to a level comparable to that of males. Overall, the consistency of age-related trends in SWS in males between visual annotation and automated labeling, as well as the lack of consistency in these trends in females, is striking. Given that the 75 µV amplitude threshold was established using data acquired primarily from young males, these results suggest that observed sex-based differences in visually annotated SWS may be artifactual rather than physiological, and a result of the 75 µV amplitude criterion. This sex-related disparity highlights the need for the American Association of Sleep Medicine guidelines for scoring SWS to be reviewed and updated to provide equivalent performance for males and females.

Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1093/sleep/zsaf063

Authors

More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Engineering Science
Role:
Author
ORCID:
0000-0002-5868-8640
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Clinical Neurosciences
Role:
Author
ORCID:
0000-0002-1683-0477
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Clinical Neurosciences
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Engineering Science
Role:
Author


Publisher:
Oxford University Press
Journal:
Sleep More from this journal
Volume:
48
Issue:
10
Article number:
zsaf063
Publication date:
2025-03-13
Acceptance date:
2025-01-27
DOI:
EISSN:
1550-9109
ISSN:
0161-8105


Language:
English
Keywords:
Pubs id:
2094512
Local pid:
pubs:2094512
Deposit date:
2025-03-20
ARK identifier:

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