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Cloning of PCP1, a member of a family of pollen coat protein (PCP) genes from Brassica oleracea encoding novel cysteine-rich proteins involved in pollen-stigma interactions.

Abstract:

The pollen coatings of both Brassica oleracea and Brassica napus contain a small family of basic 6-8 kDa proteins which are released on to the stigmatic surface on pollination. Following partial amino-acid sequencing of one of these pollen coat proteins (PCPs), PCR primers were constructed to isolate the PCP sequence from anther mRNA using RT-PCR. A cDNA was obtained which, in Northern hybridization experiments, revealed a characteristic pattern of expression during late stages of anther deve...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MPLS, Plant Sciences
Role:
Author
Journal:
The Plant journal : for cell and molecular biology
Volume:
10
Issue:
2
Pages:
303-313
Publication date:
1996-08-05
DOI:
EISSN:
1365-313X
ISSN:
0960-7412
URN:
uuid:178c294d-81f0-463d-a8ef-cccb9532948b
Source identifiers:
53221
Local pid:
pubs:53221

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