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Journal article

AAV-mediated gene transfer of human X-linked inhibitor of apoptosis protects against oxidative cell death in human RPE cells.

Abstract:

PURPOSE: To determine whether human X-linked inhibitor of apoptosis (XIAP) enhances the survival of cultured human retinal pigment epithelial cells exposed to H(2)O(2). METHODS: ARPE-19 cells were exposed to H(2)O(2) to induce oxidative cell death. Intracellular reactive oxygen species (ROS) were measured using 2',7'-dichlorofluorescein diacetate. MTT assay was performed to quantify mitochondrial stress. Cell apoptosis was determined by TUNEL assay. Human XIAP was delivered with bicistronic ...

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Publication status:
Published

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Publisher copy:
10.1167/iovs.10-6850

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Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Neuroscience
Role:
Author
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Journal:
Investigative ophthalmology and visual science
Volume:
52
Issue:
13
Pages:
9591-9597
Publication date:
2011-01-01
DOI:
EISSN:
1552-5783
ISSN:
0146-0404
URN:
uuid:176e4bec-701b-41ed-bded-47aac343895f
Source identifiers:
198754
Local pid:
pubs:198754

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