Probing the environment of neurotensin whilst bound to the neurotensin receptor by solid state NMR.

Williamson, P; Bains, S; Chung, C; Cooke, R; Watts, A

Journal article

Probing the environment of neurotensin whilst bound to the neurotensin receptor by solid state NMR.

Abstract:: A functionally active analogue of neurotensin, neurotensin(8-13), has been observed whilst bound to the agonist-binding site of the rat neurotensin receptor by nuclear magnetic resonance (NMR). Through the application of slow magic angle sample spinning and high-power proton decoupling, sufficient resolution and sensitivity were obtained in the carbon-13 spectrum to allow an assignment of many of the side chain resonances arising from uniformly carbon-13/nitrogen-15-labelled neurotensin(8-13) whilst bound to the neurotensin receptor. Significant perturbations in carbon-13 chemical shift were observed upon the binding of the neurotensin(8-13) to the receptor. Most importantly significant shifts were observed in both the carboxy terminus and tyrosine side chain of the neurotensin(8-13), suggesting that these sites are important in the interaction of the neurotensin with the agonist-binding site on the neurotensin receptor. Conversely, no perturbations were observed for the carbon-13 sites within the guanidinium groups of the arginine side chains, indicating little interaction with the receptor-binding site, or a shielding of the local environment by the surrounding nitrogen atoms. These NMR observations lend further support to previous structure-activity studies, site-directed mutagenesis and modelling studies of the agonist-binding site of the neurotensin receptor, from which the same specific residues for which NMR perturbations were observed are important for neurotensin receptor activation by neurotensin.

Publication status:: Published

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APA Style

Williamson, P., Bains, S., Chung, C., Cooke, R., & Watts, A. (2002). Probing the environment of neurotensin whilst bound to the neurotensin receptor by solid state NMR. FEBS Letters, 518(1-3), 111–115.

MLA Style

Williamson, P, et al. “Probing the Environment of Neurotensin Whilst Bound to the Neurotensin Receptor by Solid State NMR.” FEBS Letters, vol. 518, nos. 1-3, 2002, pp. 111–15.

Chicago Style

Williamson, P, S Bains, C Chung, R Cooke, and A Watts. 2002. “Probing the Environment of Neurotensin Whilst Bound to the Neurotensin Receptor by Solid State NMR.” FEBS Letters 518 (1-3): 111–15.
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