Structure of vaccinia virus thymidine kinase in complex with dTTP: insights for drug design.

Journal article

BACKGROUND: Development of countermeasures to bioterrorist threats such as those posed by the smallpox virus (variola), include vaccination and drug development. Selective activation of nucleoside analogues by virus-encoded thymidine (dThd) kinases (TK) represents one of the most successful strategies for antiviral chemotherapy as demonstrated for anti-herpes drugs. Vaccinia virus TK is a close orthologue of variola TK but also shares a relatively high sequence identity to human type 2 TK (hTK), thus achieving drug selectivity relative to the host enzyme is challenging. RESULTS: In order to identify any differences compared to hTK that may be exploitable in drug design, we have determined the crystal structure of VVTK, in complex with thymidine 5'-triphosphate (dTTP). Although most of the active site residues are conserved between hTK and VVTK, we observe a difference in conformation of residues Asp-43 and Arg-45. The equivalent residues in hTK hydrogen bond to dTTP, whereas in subunit D of VVTK, Asp-43 and Arg-45 adopt a different conformation preventing interaction with this nucleotide. Asp-43 and Arg-45 are present in a flexible loop, which is disordered in subunits A, B and C. The observed difference in conformation and flexibility may also explain the ability of VVTK to phosphorylate (South)-methanocarbathymine whereas, in contrast, no substrate activity with hTK is reported for this compound. CONCLUSION: The difference in conformation for Asp-43 and Arg-45 could thus be used in drug design to generate VVTK/Variola TK-selective nucleoside analogue substrates and/or inhibitors that have lower affinity for hTK.

Authors: El Omari, K; Solaroli, N; Karlsson, A; Balzarini, J; Stammers, D

• Publication status: Published
• Journal: BMC structural biology
• Volume: 6
• Pages: 22
• Publication date: 2006-01-01
• DOI: 10.1186/1472-6807-6-22
• EISSN: 1472-6807
• ISSN: 1471-2237
• Language: English
• Keywords: Thymidine Kinase; Drug Design; Vaccinia virus; Mutation; Binding Sites; Models, Molecular; Crystallography, X-Ray; Models, Biological; Viral Proteins; Ligands; Mutagenesis, Site-Directed; Sensitivity and Specificity; Thymine Nucleotides; Kinetics; Macromolecular Substances; Protein Binding; Molecular Conformation