Journal article
Machining protein microcrystals for structure determination by electron diffraction
- Abstract:
- We demonstrate that ion-beam milling of frozen, hydrated protein crystals to thin lamella preserves the crystal lattice to near-atomic resolution. This provides a vehicle for protein structure determination, bridging the crystal size gap between the nanometer scale of conventional electron diffraction and micron scale of synchrotron microfocus beamlines. The demonstration that atomic information can be retained suggests that milling could provide such detail on sections cut from vitrified cells.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
Actions
Access Document
- Files:
-
-
(Preview, Version of record, pdf, 890.6KB, Terms of use)
-
- Publisher copy:
- 10.1073/pnas.1809978115
Authors
+ National Institutes of Health
More from this funder
- Funding agency for:
- Chiu, W
- Grant:
- P41GM103832
+ Wellcome Trust
More from this funder
- Funding agency for:
- Duyvesteyn, H
- Kotecha, A
- Ginn, H
- Zhang, P
- Grant:
- ALR00750-B500.1
- 101122/Z/13/Z
- ALR00040
- 206422/Z/17/Z
- 090532/Z/09/Z
- Publisher:
- National Academy of Sciences
- Journal:
- Proceedings of the National Academy of Sciences More from this journal
- Volume:
- 115
- Issue:
- 38
- Pages:
- 9569-9573
- Publication date:
- 2018-08-31
- Acceptance date:
- 2018-08-07
- DOI:
- EISSN:
-
1091-6490
- ISSN:
-
0027-8424
- Pmid:
-
30171169
- Language:
-
English
- Keywords:
- Pubs id:
-
pubs:911936
- UUID:
-
uuid:1719c15d-dc16-4567-9161-f9db50b26b22
- Local pid:
-
pubs:911936
- Source identifiers:
-
911936
- Deposit date:
-
2018-09-28
Terms of use
- Copyright holder:
- Duyvesteyn, et al
- Copyright date:
- 2018
- Notes:
-
Copyright © 2018 the Author(s). Published by PNAS.
This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).
If you are the owner of this record, you can report an update to it here: Report update to this record