Journal article
SAG-RAD: a method for single-cell population genomics of unicellular eukaryotes
- Abstract:
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Sequencing of reduced representation libraries enables genotyping of many individuals for population genomic studies. However, high amounts of DNA are required, and the method cannot be applied directly on single cells, preventing its use on most microbes. We developed and implemented the analysis of single amplified genomes followed by restriction-site-associated DNA sequencing to bypass labor-intensive culturing and to avoid culturing bias in population genomic studies of unicellular eukaryotes. This method thus opens the way for addressing important questions about the genetic diversity, gene flow, adaptation, dispersal, and biogeography of hitherto unexplored species.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 1.1MB, Terms of use)
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- Publisher copy:
- 10.1093/molbev/msad095
Authors
- Publisher:
- Oxford University Press
- Journal:
- Molecular Biology and Evolution More from this journal
- Volume:
- 4
- Issue:
- 5
- Article number:
- msad095
- Publication date:
- 2023-04-20
- Acceptance date:
- 2023-04-05
- DOI:
- EISSN:
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1537-1719
- ISSN:
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0737-4038
- Language:
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English
- Keywords:
- Pubs id:
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1338297
- Local pid:
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pubs:1338297
- Deposit date:
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2023-05-02
- ARK identifier:
Terms of use
- Copyright holder:
- Gollnisch et al.
- Copyright date:
- 2023
- Rights statement:
- © The Author(s) 2023. Published by Oxford University Press on behalf of Society for Molecular Biology and Evolution. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
- Licence:
- CC Attribution (CC BY)
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